Comparison Between two Techniques HPLC, ELISA in the Estimation of Levels of Vitamin D in Plasma
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Background: Vitamin D is very important in the metabolism of calcium and phosphate in the body. The deficiency of that vitamin may cause different cases like osteoporosis, osteomalacia, cancers and etc. And because of the importance of that vitamin, there are various methods used to determine their levels in the blood. Objectives: to evaluate whether HPLC or ELISA techniques in the estimation of levels of vitamin D in plasma are better by comparison between them in accuracy and clinical implications. Material and Methods: Blood samples were taken in the private clinical laboratory (Al-Jawadeen Laboratory) for people with suspected vitamin D deficiency, their ages ranged from (20-45 years), and the samples were tested in the laboratory in an automated device for vitamin D level then the same remained samples were used to determine levels of the vitamin by ELISA and HPLC. Results: The results reveal that there were no significant differences between Laboratory results and ELISA results for vitamins. There was a significant difference between laboratory and HPLC results and significant differences between ELISA and HPLC results. Conclusions: The results of the HPLC method reveal a lower level of 25-OH-hydroxyvitamin D compared to the other techniques used here. These results need additional studies with high sample sizes to be more accurate in deciding which method is most appropriate in estimating vit D.
Keywords
Vitamin D, HPLC, ELISA, 25(OH) D