Molecular Diagnosis of Yeasts Isolated From Women with Vaginal Candidiasis
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Description
This study aimed to isolate and diagnose the fungi causing genital tract infections from married women and extract DNA from the isolated and diagnosed samples on chrome agar medium. Studying the extent of genetic variation of Candida isolates using Specific-PCR Determine the sequences of the nitrogenous bases of the products of the Specific-PCR reaction and compare them with the sequences of the standard strains present in Gen Bank to reveal the degree of similarity and to find a relationship between them and the standard strains from Gen Bank.
Keywords
Candida, ITS1, ITS4, DNA, Real-time PCR