The Effect of Antibody Concentration on Pilator Sensitivity (Rapid Salmonella Detector) as a Colourimetric-Based Salmonella Detection Biosensor
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Publishing House Education and Science s.r.o.
Abstract
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Food safety is one of the essential basics of human rights. Food should be the source of nourishment for human beings and not an open door for potentially pathogenic bacteria to avoid foodborne disease. One of the most pathogenic bacteria is Salmonella. This study aims to know the effect of antibody concentration on PILATOR (Rapid Salmonella Detector) uses the descriptive method. The results can detect Salmonella in 10 minutes, with the optimum concentration of primary and secondary antibodies being 10 μg ml-1 and 0.2 μg ml-1. Based on time detection (10 minutes) and 102 CFU/ml limit detection. PILATOR is categorized as a fast and accurate Salmonella detector.
Keywords
Biosensor, Immunosensor, PILATOR, Salmonella, Antibody